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Supplementary file, Material and methods, RT-QpCR.doc

First, the difference in the cycle threshold (Ct) values between the 18S gene and a ... using real-time quantitative PCR and the 2 ... alignment with high accuracy ...  

Supplementary Text S1: Detection of total HIV-1 DNA.doc

To ensure Ct values were within the acceptable ... The minimal inter- and intra- assay variation demonstrated the high reproducibility of the QPCR assay. References. 1 ...  

Protocol for Real-Time RT-PCR - MGH-PGA: Parabiosys -.doc

This protocol describes the detailed experimental procedure for real-time RT-PCR using SYBR Green I as mentioned in Xiaowei Wang and Brian Seed (2003) A PCR primer ...   Down

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  • Protocol for Real-Time RT-PCR - MGH-PGA: Parabiosys - …

    This protocol describes the detailed experimental procedure for real-time RT-PCR using SYBR Green I as mentioned in Xiaowei Wang and Brian Seed (2003) A PCR primer ...

    pga.mgh.harvard.edu/primerbank/PCR_protocol.doc
  • Detailed Methods: Supplementary Information

    Quantitative PCR (qPCR) qPCR reactions ... we used 132 l of high-quality cDNA extracted from 12 week R6/2 ... The Ct values for each sample were averaged to …

    www.biomedcentral.com/content/supplementary/1750-1326-3-17-S6.doc
  • Cytoplasmic RNA improves accuracy of mRNA sequencing …

    CT values for exonic and intronic expression between the cytoplasmic and polyA+ selected RNA. QPCR charts, ... The values obtained for the modified protocol were …

    www.biomedcentral.com/content/supplementary/1472...
  • Supplementary Material

    Figure S2. qPCR amplification of genomic DNA for wild ... cefG (3) for high-producing RNCM F-4081D strain; d – Ct values of amplified genes for high-producing ...

    static-content.springer.com/esm/art%3A10.1007%2Fs11274-014-1721-1/...