First, the difference in the cycle threshold (Ct) values between the 18S gene and a ... using real-time quantitative PCR and the 2 ... alignment with high accuracy ... Down
To ensure Ct values were within the acceptable ... The minimal inter- and intra- assay variation demonstrated the high reproducibility of the QPCR assay. References. 1 ... Down
Quantitative PCR (qPCR) qPCR reactions were performed in triplicate with 5 μl of diluted cDNA template in a 25 μl reaction containing 2x Precision Mastermix ... Down
Quantitative PCR (qPCR) qPCR reactions were performed in triplicate with 5 μl of diluted cDNA template in a 25 μl reaction containing 2x Precision Mastermix ...www.biomedcentral.com/content/supplementary/1750-1326-3-17-S6.doc
CT values for exonic and intronic expression between the cytoplasmic and polyA+ selected RNA. QPCR charts, ... The values obtained for the modified protocol were …www.biomedcentral.com/content/supplementary/1472-6750-13-99-s1.doc
In addition, 2 miRNAs were also evaluated by RT-QPCR: miR-204, which showed to be expressed at high levels in kidney (5) ... RT-QPCR results (delta Ct values) ...www.nature.com/ki/journal/v85/n2/extref/ki2013338x1.doc
Fix incorrect rounding for Amp Score values in Export Result and CRT/CT values in well table. ... Due to high utilization in the instrument CPU: Periodically, ...downloads.lifetechnologies.com/Instrument_Software/qPCR/Quant...