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Supplementary file, Material and methods, RT-QpCR.doc

First, the difference in the cycle threshold (Ct) values between the 18S gene and a ... using real-time quantitative PCR and the 2 ... high accuracy and high ... Read  Down

Protocol for Real-Time RT-PCR - Harvard University.doc

This protocol describes the detailed experimental procedure for real-time RT-PCR using SYBR Green I as mentioned in Xiaowei Wang and Brian Seed (2003) A PCR primer ...Read  Down

Supplementary Text S1: Detection of total HIV-1 DNA.doc

To ensure Ct values were within the acceptable ... The minimal inter- and intra- assay variation demonstrated the high reproducibility of the QPCR assay. References. 1 ... Read  Down

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  • Supplementary Text S1: Detection of total HIV-1 DNA ...

    To ensure Ct values were within the acceptable ... The minimal inter- and intra- assay variation demonstrated the high reproducibility of the QPCR assay. References. 1.

    www.plosone.org/article/fetchSingleRepresentation...
  • Biotage/ qRT-PCR notes - Montana State University

    qPCR website: http ... even in the absence of standards by examining Ct values. ... with a DNA template should result in a low Ct-value with a high increase ...

    www.montana.edu/wwwmb/uploads/researcherfiles/...
  • www.nature.com

    Right panel: RT-QPCR results (delta Ct values) ... (FDR) using the q-value package from Bioconductor (7, 8). All miRNA probe sets having a p-value <0.01, ...

    www.nature.com/ki/journal/v85/n2/extref/ki2013338x1.doc
  • Unit 6:

    Setting a threshold line for Ct analysis. In real-time qPCR, ... The Ct values for the ... Effects of initial template DNA concentration on Ct values. The Ct value, ...

    www.austincc.edu/jgotcher/Files/Intrumentation%20...
  • Accuracy Assessment of Satellite Remote Sensing for ...

    The organic extraction method is well suited for forensic samples because of the high ... This indicates that high CT values of the internal control during qPCR may ...

    csus-dspace.calstate.edu/bitstream/handle/10211.9/...
  • Read Me - Download

    Fix incorrect rounding for Amp Score values in Export Result and CRT/CT values in well table. ... For a High Resolution Melt experiment, ...

    downloads.lifetechnologies.com/Instrument_Software/...