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RT-PCR: Two-Step Protocol - MIT OpenCourseWare | Free.pdf

Step Two: PCR A) Primer Preparation 1) Primers are shipped in dry form. Briefly spin the tube before you open the cap to avoid loss of DNA pellet ...  Down

Touchdown PCR: using the decrement temperature.pdf

Touchdown PCR In touchdown PCR the annealing temperature is gradually decreased during the ... segment to edit the decrement temperatures for the annealing step ...  Down

Multiplex PCR: Critical Parameters and Step-by-Step.pdf

some loci, even though we tried to compensate using a longer annealing time and slightly longer extension time. Extension time (Step 5, A, B and ...   Down

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  • Multiplex PCR: Critical Parameters and Step-by-Step …

    some loci, even though we tried to compensate using a longer annealing time and slightly longer extension time. Extension time (Step 5, A, B and

    www.biotechniques.com/multimedia/archive/00011/97233rr01_11727a.pdf
  • Real­time PCR Master Mix - Fisher

    Real­time PCR Master Mix ... increase extension time to generate longer PCR products. NOTE: Shorter annealing step time (<10sec.) can be used for amplicon <100bp.

    www.fishermolecularbiology.com/uploads/files/2014-04-07_Real%20...
  • PCR Optimization: Reaction Conditions and Components

    Therefore, primer extension will occur during the annealing step and the hybrid ... be completely or partially activated in a pre-PCR heat step (conventional Hot-

    www3.appliedbiosystems.com/cms/groups/mcb_marketing/documents/...
  • Performing Fast PCR - Bio-Rad Laboratories

    A two-step PCR protocol was used ... polymerase requires an annealing temperature 5°C above typical annealing temperatures, two-step protocols often can be run ...

    www.bio-rad.com/LifeScience/jobs/2005/05-0739/fast_pcr.pdf