Auble Lab Northern Blots (based on protocol from Mitch Smith Lab) Use gel apparatus designated for RNA. Wipe apparatus with “RNase Away”. 1% Agarose gel (100ml) ...
The specific contents of each lane on the Northern Blot are described on the Certificate of Analysis Sheet included with your order. The OriGene Northern Blot is ...
Sisson lab last updated 06/28/07 miRNA Northern Blot Adapted from McManus Lab Protocol (MIT) Reagents 30% Acrylamide/Bis 29:1 (BioRad #161-0156) ... Down
Sisson lab last updated 06/28/07 miRNA Northern Blot Adapted from McManus Lab Protocol (MIT) Reagents 30% Acrylamide/Bis 29:1 (BioRad #161-0156)www.utexas.edu/.../protocols/molecularbiology/miRNA_Northern_Blot.pdf
7) Calculating concentration: -For Northern Blots, load 15ug per well if possible. For soleus, 8ug per well is usually plentyÆ *take your A260 values for a sample ...kellylab.wustl.edu/protocols/RNA_and_northern_blot3-3-2006.pdf
Odyssey ® Northern Blot Analysis Page 5 www.licor.com Doc# 988-09394 Denature Probe Hybridization Stringency Washes V. Biotin Detection for Northern Blotswww.users.drew.edu/jliu3/Docs/LICOR Northern.pdf
Following exposure of a Northern or Southern blot ... blot will become permanently bound once the blot is dried. Test for Stripping Efficiencyhttps://tools.lifetechnologies.com/.../TR0028-Strip-Southern-blots.pdf