Auble Lab Northern Blots (based on protocol from Mitch Smith Lab) Use gel apparatus designated for RNA. Wipe apparatus with “RNase Away”. 1% Agarose gel (100ml) ... Down
Sisson lab last updated 06/28/07 miRNA Northern Blot Adapted from McManus Lab Protocol (MIT) Reagents 30% Acrylamide/Bis 29:1 (BioRad #161-0156) ... Down
7) Calculating concentration: -For Northern Blots, load 15ug per well if possible. For soleus, 8ug per well is usually plentyÆ *take your A260 values for a sample ... Down
7) Calculating concentration: -For Northern Blots, load 15ug per well if possible. For soleus, 8ug per well is usually plentyÆ *take your A260 values for a sample ...kellylab.wustl.edu/protocols/RNA_and_northern_blot3-3-2006.pdf
The specific contents of each lane on the Northern Blot are described on the Certificate of Analysis Sheet included with your order. The OriGene Northern Blot is ...www.origene.com/assets/Documents/catalog_manual/AppGuideNorthern...
Northern Blot Analysis Using Biotin-Labeled Probes Published January 2006. Revised June 2012. The most recent version of this pack insert is posted atbiosupport.licor.com/docs/Northern_09394.pdf
1 Northern Blot Use this protocol if you have hybridization bottles. • Hybridization of DIG labeled (non-radioactive) DNA probe to a nylon membrane blotted with RNA.faculty.unlv.edu/aandres/pdf/protocols/northern_bottles.pdf